Abstract
WE have shown1 that diphtheria toxin with a value of 150–200 flocculating units per ml. can be obtained by growing the Park–Williams No. 8 strain of C. diphtheriae as a submerged culture, using a tryptic digest medium based on that described elsewhere2. The volumes employed were 10–14 litres of medium, and the vessels were of glass, with stirrers made of either ‘Birmabright’ (a magnesium–aluminium alloy) or aluminium.
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References
Linggood, F. V., Matthews, A. C., Pinfield, S., Pope, C. G., and Sharland, T. R., Nature, 174, 557 (1954).
Linggood, F. V., and Fenton, E. L., Brit. J. Exp. Path., 28, 354 (1947).
Chain, E. B., Paladino, S., Callow, D. S., Ugolini, F., and Van Der Sluis, J., Bull. World Health Org., 6, 73 (1952).
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LINGGOOD, F., MATTHEWS, A., PINFIELD, S. et al. Production of Diphtheria Toxin in Submerged Culture. Nature 176, 1128 (1955). https://doi.org/10.1038/1761128a0
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DOI: https://doi.org/10.1038/1761128a0
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