Abstract
WHEN bull semen is diluted in a buffered (pH. 7.0) solution of sodium chloride which is isotonic with bull seminal plasma (Δ, − 0.54° C.) and is filtered through a layer of small glass balls, the dead spermatozoa are retained by the filter while live ones pass through; it is therefore possible to estimate the percentage dead spermatozoa in bull semen by comparing the opacity of diluted semen before and after filtration.
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References
Campbell, R. C., Hancock, J. L., and Rothschild, Lord, J. Exp. Biol., 30, 44 (1953).
Bishop, M. W. H., Campbell, R. C., Hancock, J. L., and Walton, A., J. Agric. Sci., 44, 227 (1954).
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BANGHAM, A., HANCOCK, J. A New Method for counting Live and Dead Bull Spermatozoa. Nature 176, 656 (1955). https://doi.org/10.1038/176656a0
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DOI: https://doi.org/10.1038/176656a0
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