Abstract
FOLLOWING on work in which much indirect evidence implicated the plasma-cell as the site of antibody production1, microscopic demonstration of antibody in plasma-cells was effected by a fluorescein-conjugate technique2. However, this method is limited to some extent by the difficulty in studying the morphology of antibody-containing cells, by the possibility of non-specific staining and by its inability to yield quantitative results. Further, since this technique renders both antibody and antigen in tissues fluorescent, it would be difficult to exclude the presence of a small amount of antibody in the neighbourhood of persisting injected antigen.
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BERENBAUM, M. Autoradiographic Localization and Measurement of Intracellular Antibody, using Labelled Antigen. Nature 177, 46–47 (1956). https://doi.org/10.1038/177046a0
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DOI: https://doi.org/10.1038/177046a0
