Spectrophotometric Method for the Estimation of Vitamin K₃

Abstract

Reddy and Srinivasan¹ have described a method for the estimation of vitamin K and vitamin K₃ which is slightly different from that of Novelli². During the course of our investigation on the biosynthesis of vitamin K in moulds, we found that the method of Reddy and Srinivasan was not suitable for determining microquantities of vitamin K. It was observed that, on standing, the intensity of the colour at the junction of the two layers increases, with the result that the intensity of colour is not uniform throughout the layer. In the present communication, we wish to describe a modified method which can be used to estimate vitamin K in the range 2–30 µgm.