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Solubility of Albumin in Alcohol after Precipitation by Trichloroacetic Acid: a Simplified Procedure for Separation of Albumin

Nature volume 178, page 1067 (1956)Cite this article

Abstract

RADIOACTIVE proteins are prepared for counting in many laboratories1–3 by slight modifications of the basic technique of precipitation with 10 per cent trichloroacetic acid, several washes with 5 or 10 per cent trichloroacetic acid, treatment with hot alcohol, hot alcohol–ether and drying with ether. When it is required to remove nucleic acids, the protein is treated with 5 per cent trichloroacetic acid at 90° for 15 min. before the hot alcohol wash.

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Author notes

  1. A. KORNER

    Present address: Department of Biochemistry, University of Cambridge, Cambridge, England

  2. A. KORNER: Rockefeller Foundation Fellow 1955–56

Authors and Affiliations

  1. Department of Physiological Chemistry, University of California School of Medicine, Berkeley, California

    A. KORNER & J. R. DEBRO

Authors
  1. A. KORNER
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  2. J. R. DEBRO
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KORNER, A., DEBRO, J. Solubility of Albumin in Alcohol after Precipitation by Trichloroacetic Acid: a Simplified Procedure for Separation of Albumin. Nature 178, 1067 (1956). https://doi.org/10.1038/1781067a0

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