Use of Plastic Tubing in Chromatography

Abstract

DURING a chromatographic separation of the carotenoid pigments of the starfish Marthasterias glacialis (L.) from Madeira, it was found that some pigment disappeared during elution, which was necessarily prolonged in order satisfactorily to separate the relatively large number of pigments present in the total carotenoid extract. Several pigments were lost in this way (presumably owing to oxidation on the column), and further, some of the zones were so small as to make it difficult to isolate them by extruding the column.