Activation of Amino-acids by Soluble Enzymes from Pancreas and Other Tissues

Abstract

LAST year, Hoagland¹ reported that he had obtained from rat liver a soluble enzyme preparation which catalysed the formation of amino-acid hydroxamates when incubated with a mixture of twelve amino-acids in the presence of adenosine triphosphate and hydroxylamine. He suggested that this type of carboxyl group activation might be a first step in protein synthesis. In a later paper with Keller and Zamecnik² he reported partial purification of activating enzyme from liver.