Abstract
WHEN diaphragm muscle is suspended in oxygenated Krebs solution at 38° C., it remains viable for many hours. Analysis, however, has shown that prolonged immersion of the muscle in physiological saline is associated with a small loss of potassium from the tissue and a considerable increase in sodium1. Preliminary experiments suggested that this increase in tissue sodium could be largely avoided if the muscle was suspended in rat serum instead of in saline. Later, reconstituted human freeze-dried serum was found to be as effective as rat serum, and has been used in the experiments reported here.
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CREESE, R., D'SILVA, J. & NORTHOVER, J. Effect of Insulin on Sodium in Muscle. Nature 181, 1278 (1958). https://doi.org/10.1038/1811278a0
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DOI: https://doi.org/10.1038/1811278a0
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