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Autoradiographic Visualization of Synthesis of Deoxyribonucleic Acid in Tissue Culture with Tritium-labelled Thymidine

Abstract

THYMIDINE is a specific precursor of deoxyribonucleic acid1. The very low energy of tritium electrons permits precise localization on autoradiographs (within less than one micron on average) of the atom that disintegrates. This is not possible with other radioisotopes2 such as phosphorus-32 or even carbon-14 or sulphur-35.

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FIRKET, H., VERLY, W. Autoradiographic Visualization of Synthesis of Deoxyribonucleic Acid in Tissue Culture with Tritium-labelled Thymidine. Nature 181, 274–275 (1958). https://doi.org/10.1038/181274a0

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