Abstract
RATOON stunting, a virus disease of sugar cane, is difficult to diagnose since the macroscopic symptoms are ill defined. Several tests described for diagnosing virus diseases in plants, including one for sugar cane1, were tried but gave inconsistent results. Attempts were therefore made to develop a colour reaction for detecting the presence of the virus. The investigations were prompted by reports on the use of 2,3,5-triphenyl tetrazolium chloride to detect viruses in plants2 and on the use of the same substance to localize the site of reducing activity in sugar cane3.
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References
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Hughes, C. G., and Steindl, D. R. L., Tech. Com. No. 2 Bureau of Sugar Experiment Stations, Queensland (1955).
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ANTOINE, R. A Staining Technique for detecting Ratoon Stunting Disease in Sugar Cane. Nature 181, 276–277 (1958). https://doi.org/10.1038/181276a0
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DOI: https://doi.org/10.1038/181276a0
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