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Demonstration of Some ‘Masked’ Lipids in the Oocytes of Chrotogonus trachypterus with Sudan Black B and Phenol ‘Unmasking’ Techniques

Abstract

SUDAN black B, in spite of being the most specific and vigorous dye for the demonstration of lipids in tissue sections1,2, has its limitations3,4. The cell inclusion believed to be lipid may not be coloured with sudan black B, because: (1) some lipids (like phospholipids) may escape from the tissue into the fixative by the process of emulsification2,3; (2) the lipids present have a high melting point and thus remain ‘solid’ in tissue sections3; or (3) the lipids present are ‘masked’ by proteins or other substances.

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GUPTA, B. Demonstration of Some ‘Masked’ Lipids in the Oocytes of Chrotogonus trachypterus with Sudan Black B and Phenol ‘Unmasking’ Techniques. Nature 181, 555 (1958). https://doi.org/10.1038/181555a0

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