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A Sensitive Micro-Spectrophotometric Method for Succinate Analysis

Abstract

ASSAY of succinic acid by estimation of reduction of a redox dye in a succinic dehydrogenase and cyanide–poisoned system has been reported by Lardy1 for methylene blue, and by Vishniac and Ochoa2 for 2,6-dichloroindophenol. The disadvantage to the use of these dyes is that they are non-enzymatically reoxidized directly by oxygen and there is uncertainty concerning the end-point of the reduction of the dye and thus about the amount of succinate oxidized.

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References

  1. Lardy, H. A., edit., “Respiratory Enzymes” (Burgess, Minneapolis, 1949).

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  2. Vishniac, W., and Ochoa, S., J. Biol. Chem., 195, 75 (1952).

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  3. Umbreit, W. W., Burris, R. H., and Stauffer, J. F., “Manometric Techniques”, 3rd edit. (Burgess, Minneapolis, 1957).

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WAITZMAN, M., ADAMS, E. A Sensitive Micro-Spectrophotometric Method for Succinate Analysis. Nature 182, 129 (1958). https://doi.org/10.1038/182129a0

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