Abstract
ASSAY of succinic acid by estimation of reduction of a redox dye in a succinic dehydrogenase and cyanide–poisoned system has been reported by Lardy1 for methylene blue, and by Vishniac and Ochoa2 for 2,6-dichloroindophenol. The disadvantage to the use of these dyes is that they are non-enzymatically reoxidized directly by oxygen and there is uncertainty concerning the end-point of the reduction of the dye and thus about the amount of succinate oxidized.
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References
Lardy, H. A., edit., “Respiratory Enzymes” (Burgess, Minneapolis, 1949).
Vishniac, W., and Ochoa, S., J. Biol. Chem., 195, 75 (1952).
Umbreit, W. W., Burris, R. H., and Stauffer, J. F., “Manometric Techniques”, 3rd edit. (Burgess, Minneapolis, 1957).
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WAITZMAN, M., ADAMS, E. A Sensitive Micro-Spectrophotometric Method for Succinate Analysis. Nature 182, 129 (1958). https://doi.org/10.1038/182129a0
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DOI: https://doi.org/10.1038/182129a0