Abstract
GIRARD'S reagent T (trimethylammonium hydrazide chloride)1 is extensively used for the separation of oxo-steroids from other steroids and particularly for purification of 17-oxo-steroids in extracts of acid- or enzyme-hydrolysed urine2. The method employed usually involves dissolving the material and Girard reagent in glacial acetic acid, and either heating the mixture for periods of up to 1 hr. or allowing it to stand overnight at room temperature. During this procedure destruction of labile steroids and some acetylation of hydroxy-steroids tend to occur (ref. 3 and unpublished observations).
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References
Girard, A., and Sandulesco, G., Helv. Chim Acta, 19, 1095 (1936).
Pincus, G., and Pearlman, W. H., Endocrinol., 29, 413 (1941).
Brooks, R. V., Biochem. J., 68, 50 (1958).
Teitelbaum, C. L., J. Org. Chem., 23, 646 (1958).
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TAYLOR, W. Isolation of Oxo-Steroids under Neutral Conditions. Nature 182, 1735 (1958). https://doi.org/10.1038/1821735a0
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DOI: https://doi.org/10.1038/1821735a0
