Abstract
BY the use of rectangular strips of filter paper, rather than filter paper disks, in combination with a horizontal solvent flow at an elevated temperature, amino-acids are rapidly separated as round compact spots rather than circular bands1. This is desirable since such spots in many cases lend themselves to quantitative study by the maximum colour-density technique. Recently, such a procedure has been applied to the analysis of tryptophan in an alkaline hydrolysate of α-casein2.
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References
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Roberts, H. R., and Kolor, M. G., Anal. Chem., 29, 1800 (1957).
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ROBERTS, H., KOLOR, M. Rapid Quantitative Horizontal Paper Chromatography at an Elevated Temperature: Determination of Aspartic and Glutamic Acids. Nature 183, 460–461 (1959). https://doi.org/10.1038/183460a0
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DOI: https://doi.org/10.1038/183460a0
