Abstract
INADEQUATE functional ‘survival’ of hearts cultured in supernatant containing serum with a high potassium-level from lysed red cells led us to investigate the effects of supernatant potassium-levels on the electrical activity of 1,222 hearts in quantitative tissue culture. The criterion for ‘adequate survival’ is an ability to produce potentials of at least 0.02 mV. during a minimum of three periods of observation per day (8 a.m., 1 p.m. and 5 p.m.). The apparatus, etc., is described in previous papers1. The supernatants are 0.25 per cent solutions of human serum protein in balanced salt solutions—however, they differ in potassium-level. The ‘adequate survival’ time is given to the nearest day using the smallest figure in cases of doubt.
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References
Cunningham and Estborn, Lab. Invest., 7, 156 (1958). Estborn, Cunningham et al., ibid., 7, 524 (1958).
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CUNNINGHAM, A., KLEUTCH, K. & HERBST, W. Effect of Supernatant Potassium-Level on Cardiac Activity in Quantitative Tissue Culture. Nature 186, 241–242 (1960). https://doi.org/10.1038/186241b0
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DOI: https://doi.org/10.1038/186241b0
