Abstract
Bottura and Ferrari1 have described recently a rapid technique for the cytological preparation of human chromosomes, which involves intravenous injections of deacetylmethylcolchicine 2 hr. prior to a sternal marrow puncture. The marrow is then treated with hypotonic citrate, hydrolysed and stained. As they rightly point out, there are some advantages in this rapid technique over the short- or long-term tissue culture.
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Bottura, C., and Ferrari, I., Nature, 186, 904 (1960).
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KINLOUGH, M., ROBSON, H. & HAYMAN, D. A Simplified Method for the Study of Chromosomes in Man. Nature 189, 420 (1961). https://doi.org/10.1038/189420a0
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DOI: https://doi.org/10.1038/189420a0
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