Effect of L-Glutamic Acid on the Weight of Embryonic Chick Tibiotarsi cultivated in vitro

Abstract

IN a previous communication¹ the dose-response line relating the final length of embryonic chick tibiotarsi cultivated in vitro and the concentration of L-glutamine in the medium was described. Furthermore, it was shown that the presence of 25 mgm. per 100 ml. L-glutamic acid in the medium had no effect on the position of the L-glutamine dose-response line. From this result it was argued that L-glutamic acid does not act as a precursor for L-glutamine in this system. The purpose of the present communication is to present additional results on the wet and dry weights of the same bones. These results were not included in the first communication since their discussion was irrelevant to the point at issue, and could have created confusion.