Abstract
UNTIL now the separation of cholesteryl esters and triglycerides has been performed by column1 or paper chromatography2–7. We have pointed out8 the progress of thin-layer chromatography in recent years. This method has certain advantages especially for analysis of lipids. In order to investigate the possibilities of this technique in the separation of cholesteryl esters and triglycerides we tried to separate these groups isolated from various biological sources. We have used the partition principle on the impregnated layers since the adsorption principle does not allow satisfactory separation of individual members within homological classes9–12.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on SpringerLink
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Wren, J. J., J. Chromatog., 4, 173 (1960).
Noda, M., Sci. Rep. Kyoto Prefectural Univ. Agric., 11, 169 (1959).
Kaufmann, H. P., and Makus, Z., Fette-Seifen-Anstrichmittel, 61, 631 (1959).
Kaufmann, H. P., and Schnurbusch, H., Fette-Seifen-Anstrichmittel, 61, 523 (1959).
Kuksis, A., and Beveridge, J. M. R., Canad. J. Biochem. Physiol., 38, 95 (1960).
Michalec, Č., Biochim. Biophys. Acta, 19, 187 (1956).
Michalec, Č., and Strašek, J., J. Chromatog., 4, 254 (1960).
Michalec, Č., Chem. listy, 55, 953 (1961).
Kaufmann, H. P., and Mamus, Z., Fette-Seifen-Anstrichmittel, 62, 1014 (1960).
Malins, D. C., and Mangold, H. K., J. Amer. Oil Chem. Soc., 37, 576 (1960).
Van Dam, M. J. D., De Kleuver, G. J., and De Heus, J. G., J. Chromatog., 4, 26 (1960).
Jatzkewitz, H., and Mehl, E., Z. physiol. Chem., 320, 251 (1960).
Machata, G., Mikrochim. Acta, 79 (1960).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
MICHALEC, Č., ŠULC, M. & MĚŠŤAN, J. Analysis of Cholesteryl Esters and Triglycerides by Thin-Layer Chromatography. Nature 193, 63–64 (1962). https://doi.org/10.1038/193063a0
Issue date:
DOI: https://doi.org/10.1038/193063a0
This article is cited by
-
Dünnschichtchromatographische Lipoidmuster vom normalen menschlichen Serum und Liquor cerebrospinalis
Klinische Wochenschrift (1966)
-
Thin‐layer chromatographic separation of dimethylphosphatidates derived from lecithins
Lipids (1966)
-
Quantitative estimation of isomeric monoglycerides by thin‐layer chromatography
Journal of the American Oil Chemists' Society (1965)
-
Thin‐layer chromatography of lipids
Journal of the American Oil Chemists' Society (1964)
-
The quantitative analysis of triglyceride mixtures by thin layer chromatography on silica impregnated with silver nitrate
Journal of the American Oil Chemists' Society (1963)