Abstract
A SERIES of experiments were undertaken to establish the optimal conditions for extraction of soluble fractions containing proteolytic enzymes from normal and tumour (crown-gall) tissues of Nicotiana tabacum var. Wisconsin, and for assaying the proteolytic activity of these crude stem extracts. It was hoped that the results attained would expedite the selection of a single set of conditions for future extractions of crude plant material for the purpose of purifying the proteolytic principle.
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References
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GAINOR, C., CRISLEY, F. Factors affecting Proteolytic Activity of Extracts from Normal and Tumour Tissues of Tobacco. Nature 193, 1076–1077 (1962). https://doi.org/10.1038/1931076a0
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DOI: https://doi.org/10.1038/1931076a0