Homotransplant Antigens : Preparation of Active Cellular Fractions by a Modified Method ; Properties and Attempted Dose Response

Abstract

IN an earlier communication¹, we described a method for the preparation of homotransplant antigens from the splenic cells of inbred mice. The observation that the antigenic fraction contained in the sediment (‘test sediment’) retained its activity after suspension in 2 M sodium chloride provides the basis for the development of a new method which is described here. The potencies of the new preparation are equivalent to those of the test sediment described earlier, but the new procedure is simpler and quicker than that previously reported. Other recent reports of methods of preparation of transplant antigens include those of Castermans² and Manson et al.³.