Abstract
A NUMBER of methods have been used to record photographically the fluorescence of various substances present on paper chromatograms when excited by ultra-violet light. Markham and Smith1–3 used a system consisting of a quartz mercury vapour lamp and a set of two filters containing chlorine gas and a solution of NiSO4.7H2O and CoSO4.7H2O, respectively. Bernasconi, et al.4 used a mercury vapour lamp, a red-purple filter, a cobalt sulphate and nickel sulphate filter, and a 3 mm. thick piece of ‘Plexiglass’. Abelson5 used an ultra-violet hand lamp with a Kodak 2A gelatin filter enclosed in glass plates.
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References
Markham, R., and Smith, J. D., Biochem. J., 45, 294 (1949).
Smith, J. D., and Markham, R., Biochem. J., 46, 509 (1950).
Markham, R., and Smith, J. D., Nature, 163, 250 (1949).
Bernasconi, R., Sigg, H. P., and Reichstein, T., Helv. Chim. Acta, 38, 1767 (1955).
Abelson, D., Nature, 188, 850 (1960).
Cox, R. I., Nature, 189, 638 (1958).
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HERMAN, R., CLAYTON, L. & BRUTON, J. Preparation of Ultra-Violet Fluorescent Photographs of Steroids. Nature 193, 169–170 (1962). https://doi.org/10.1038/193169a0
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DOI: https://doi.org/10.1038/193169a0
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