Abstract
IN several experiments in which arginine uniformly labelled with carbon-14 was fed to various tissues of pines, one unidentified compound was found to contain most of the radioactivity incorporated into alcohol-soluble products. When tissue extracts were cleared for chromatography following the ion-exchange procedure of Plaisted1, the unknown was eluted from ‘Dowex 50’ resin with the acidic and neutral amino-acids. However, the RF of the unidentified compound on paper chromatography did not correspond with the RF of any common amino-acid. Subsequent tests indicated that the radioactive unknown was γ-guanidinobutyric acid, previously found in several species of higher plants, as well as in insects and mammals, by Irreverre et al.2.
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References
Plaisted, P. H., Contrib. Boyce Thompson Inst., 19, 231 (1958).
Irreverre, F., Evans, R. L., Hayden, A. R., and Silber, R., Nature, 180, 704 (1957).
Benson, A. A., Bassham, J. A., Calvin, M., Goodale, T. C., Haas, V. A., and Stepka, W., J. Amer. Chem. Soc., 72, 1710 (1950).
Rivard, D. E., and Carter, H. E., J. Amer. Chem. Soc., 77, 1260 (1955).
Thoai, N., Roche, J., and Robin Yvonne, C.R. Acad. Sci., Paris, 235, 832 (1952).
Pisano, J. J., Mitoma, C., and Udenfriend, S., Nature, 180, 1125 (1957).
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BARNES, R. Formation of γ-Guanidinobutyric Acid in Pine Tissues. Nature 193, 781 (1962). https://doi.org/10.1038/193781a0
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DOI: https://doi.org/10.1038/193781a0
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