Abstract
IN the past, demonstration of carbohydrate metabolism within tissues or cell cultures have been investigated by means of (1) utilization or accumulation of metabolites from tissue fragments in vitro or from cells propagated in suspension or monolayer cultures, and (2) enzyme-containing extracts derived from cell or tissue homogenates. Investigators1 recently described a system in which glucose-6-phosphate dehydrogenase was measured in air-dried, intact yeast cells by suspending these cells in 70 per cent glycerol. This simplified technique permits direct spectrophotometric measurement of triphosphopyridine nucleotide reduction at the 340-mµ wave-length. Application of this whole cell-glycerol suspension technique was used in the present investigation.
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SLOTNICK, V. Carbohydrate Metabolism within Intact Tissue-cultured Cells. Nature 193, 876–877 (1962). https://doi.org/10.1038/193876a0
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DOI: https://doi.org/10.1038/193876a0
