Abstract
GLYCEROL has been used as a protective agent for frozen bacteria1,2, Leptospirœ3, trypanosomes4, Toxoplosma gondii5, Entamoeba histolytica6, animal tissues7, bull spermatozoa4, and yeasts8. Although much work has been done on a variety of micro-organisms, little or no information is available regarding the protective action of glycerol on frozen Shigella sonnei. The purpose of the present investigation was to determine the effect of glycerol on frozen S. sonnei and also the effect of glycerol on cells frozen and thawed repeatedly. Four strains were studied: strain CH, obtained from Dr. Horace M. Gezon, University of Pittsburgh, Pittsburgh, Penna.; strains B–2501–3 and B–2506–3, obtained from the National Naval Medical Center, Bethesda, Md.; and strain 13–3, obtained from Dr. Arthur Abrams, Walter Reed Army Institute of Research, Washington, D.C.
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NAKAMURA, M., FARNUM, J. & OKE, M. Protective Action of Glycerol in the Freezing of Shigella sonnei. Nature 194, 405 (1962). https://doi.org/10.1038/194405a0
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DOI: https://doi.org/10.1038/194405a0
