Abstract
IN experiments where we sought a suitable adsorbent for the separation of corticoids by partition chromatography, we found that in calcium sulphate columns a good separation is obtained of less-polar corticoids when chloroform is used for elution. Based on this fact, a detailed analytical procedure was worked out for the fractionation of some corticoids in urinary extracts. The desirable properties of calcium sulphate were applied successfully in a method using thin layers, which substantially speeded up and simplified chromatographic analysis of steroid mixtures and separated the polar 17-OH-corticoids.
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References
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MATIS, J., ADAMEC, O. & GALVÁNEK, M. Use of a Thin Layer of Calcium Sulphate for the Chromatographic Separation of Corticoids. Nature 194, 477–478 (1962). https://doi.org/10.1038/194477b0
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DOI: https://doi.org/10.1038/194477b0
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