Abstract
IN using Coons's fluorescent antibody technique1,2 for the localization of antigens in tissues, difficulty may be encountered in preserving the sections cut from fresh-frozen material. Although it is customary to dry the sections on to a coverslip in a current of air before staining, dehydration of the sections during storage for several days at the low temperature of the cryostat can cause excessive distortion of the tissue and loss of immunological reactivity of the antigen.
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References
Coons, A. H., Creech, H. J., Jones, R. N., and Berliner, E., J. Immunol., 45, 159 (1942).
Coons, A. H., and Kaplan, M. H., J. Exp. Med., 91, 1 (1950).
Richards, A. G., Anderson, T. F., and Hance, R. T., Proc. Soc. Exp. Biol. (N.Y.), 51, 148 (1942).
Carsten, M. E., Arch. Path. (Chicago), 44, 96 (1947).
Blank, H., and McCarthy, P. L., J. Lab. Clin. Med., 36, 776 (1950).
Steedman, H. F., Nature, 156, 121 (1945).
McClelland, C. P., and Bateman, R. L., Chem. Eng. News, 23, 247 (1945).
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GEORGE, W., WALTON, K. Preservation of Tissue Sections in Coons's Fluorescent Antibody Technique. Nature 194, 693 (1962). https://doi.org/10.1038/194693a0
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DOI: https://doi.org/10.1038/194693a0
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