Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter
  • Published:

Effect of Alloxan on the Insulin Content of Micro-dissected Mammalian Pancreatic Islets

Nature volume 195, pages 388–389 (1962)Cite this article

Abstract

THE development of techniques for dissection and weighing of µgm. pieces of tissues1 has provided a method for the isolation of frozen dried sections of mammalian islets. The availability of a very sensitive bioassay method for insulin2 has permitted a direct assay of the insulin content of individual islets. We have undertaken the examination of the insulin content of micro-dissected islets from normal and diabetic rats at various times after alloxan injection.

Access through your institution
Buy or subscribe

This is a preview of subscription content, access via your institution

Access options

Access through your institution

Buy this article

  • Purchase on SpringerLink
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Lowry, O. H., J. Histochem. and Cytochem., 1, 420 (1953).

    Article  CAS  Google Scholar 

  2. Renold, A. E., Martin, D. B., Dagenais, Y. M., Steinke, J., Nickerson, R., and Sheps, M. D., J. Clin. Invest., 39, 1487 (1960).

    Article  CAS  Google Scholar 

  3. Williamson, J. R., and Lacy, P. E., Fed. Proc., 20, 191 (1961).

    Google Scholar 

  4. Bonting, S. L., and Mayron, B. R., Microchem. J., 5, 31 (1961).

    Article  CAS  Google Scholar 

  5. Best, C. H., Haist, R. E., and Ridout, J. H., J. Physiol., 97, 107 (1939). Fraenkel-Conrat, H. L., Herring, V. V., Simpson, M. E., and Evans, H. M., Amer. J. Physiol., 135, 404 (1941–42). Haist, R. E., and Pugh, E. J., ibid., 152, 36 (1948). Baker, D. G., and Ashworth, M. A., ibid., 192, 597 (1958).

    Article  CAS  Google Scholar 

  6. Ridout, J. H., Ham, A. W., and Wrenshall, G. A., Science, 100, 57 (1944). Wrenshall, G. A., Collins-Williams, J., and Hartroft, W. S., Amer. J. Physiol., 156, 100 (1949).

    Article  ADS  CAS  Google Scholar 

  7. Watkins, D., Cooperstein, S. J., and Lazarow, A., Biol. Bull., 121, 412 (1961).

    Google Scholar 

  8. Lacy, P. E., and Williamson, J. R., Diabetes, 11, 101 (1962).

    CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Anatomy, University of Minnesota, Minneapolis, 14

    P. K. DIXIT, I. LOWE & A. LAZAROW

Authors
  1. P. K. DIXIT
    View author publications

    Search author on:PubMed Google Scholar

  2. I. LOWE
    View author publications

    Search author on:PubMed Google Scholar

  3. A. LAZAROW
    View author publications

    Search author on:PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

DIXIT, P., LOWE, I. & LAZAROW, A. Effect of Alloxan on the Insulin Content of Micro-dissected Mammalian Pancreatic Islets. Nature 195, 388–389 (1962). https://doi.org/10.1038/195388a0

Download citation

  • Issue date:

  • DOI: https://doi.org/10.1038/195388a0

This article is cited by

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing