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Preservation of Viable Leucocytes in Glycerol at −80° C.

Nature volume 195, pages 610–611 (1962)Cite this article

Abstract

SINCE the demonstration by Polge et al.1 of the protective effect of glycerol on fowl spermatozoa stored at low temperatures, a number of mammalian tissues, including erythrocytes and bone marrow cells of man, have been successfully preserved in this manner2–4. With the recent interest in the culture of leucocytes from peripheral blood in determining human karyotypes, it would be desirable to be able to preserve leucocytes at low temperatures for later study or to permit shipment over long distances.

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Authors and Affiliations

  1. Department of Pathology, Harvard Medical School, and James Homer Wright Pathology Laboratories, Massachusetts General Hospital, Boston

    LEONARD ATKINS

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  1. LEONARD ATKINS
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ATKINS, L. Preservation of Viable Leucocytes in Glycerol at −80° C.. Nature 195, 610–611 (1962). https://doi.org/10.1038/195610a0

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