Abstract
INCREASED resolution of many components of complex biological mixtures has been accomplished by the use of substituted cellulose compounds in chromatography. Calf lens proteins have been fractionated into ten components on DEAE (diethylamino-ethyl-cellulose) by Spector1. Wheat glutin was fractionated on carboxymethyl-cellulose columns into four components by Woychick et al.2. DEAE columns were used to purify β-glucosidase from yeast by Duerkson and Halvorson3. Changes in the elution pattern of proteins of white potato tubers infected with Phytophthora infestans were found when extracts were chromatographed on DEAE4.
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Dickson, J., LORDS, J. Fractionation of Nitrogenous Compounds from Corn Leaves. Nature 196, 1099–1100 (1962). https://doi.org/10.1038/1961099a0
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DOI: https://doi.org/10.1038/1961099a0
