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Urinary 3-Methoxy-4-Hydroxymandelic Acid Excretion and Serum Electro-phoretic Patterns in the Normal and Bloated Bovine

Abstract

WORK has been in progress here since 1956 to determine if a metabolic difference exists between normal bovines and those subject to tympanites (bloat). Serum epinephrine was first measured on normal and bloated animals by the method of Weil-Malherbe and Bone1, since a difference in rate of metabolism was suspected. Although the trend for normal cattle to have a higher level of epinephrine was apparent, the results were largely inconclusive, owing to excessive variation among animals. The epinephrine averaged 2.35 and 2.03 µg/100 ml. whole blood for 27 normal and 13 bloated cattle, respectively.

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References

  1. Weil-Malherbe, H., and Bone, A. O., Biochem. J., 51, 311 (1952).

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  2. Sunderman, jun., F. W., Cleveland, P. D., Law, N. C., and Sunderman, F. W., Amer. J. Clin. Path., 34 (4), 293 (1960).

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  3. Fister, H. J., Manual of Standardized Procedures of Spectrophotometric Chemistry, C-43a, 1 (1950).

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  4. Beckman–Spinco Division, Serum Protein Analysis, Procedure B Tech. Bull. No. TB 6050A (1958).

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DYER, I., HAM, W. & LARSON, M. Urinary 3-Methoxy-4-Hydroxymandelic Acid Excretion and Serum Electro-phoretic Patterns in the Normal and Bloated Bovine. Nature 196, 392 (1962). https://doi.org/10.1038/196392a0

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