Abstract
IN a previous communication, a method for the quantitative isolation of nuclei from HeLa cells was described1. The nuclei were isolated by stirring the cells in a dilute solution of ‘Tween 80’ under controlled conditions. It seems worth reporting that the method has now been successfully applied to strain L, Chinese hamster, Ehrlich ascites and Krebs 2 ascites tumour cells. It appears likely that it will prove to be applicable to a wide range of animal cell suspensions.
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References
Fisher, H. W., and Harris, H., Proc. Roy. Soc., B, 156, 521 (1962).
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FISHER, H., HARRIS, H. Isolation of Nuclei from Animal Cell Suspensions. Nature 196, 910 (1962). https://doi.org/10.1038/196910a0
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DOI: https://doi.org/10.1038/196910a0
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