Abstract
THE glycosidic link can, theoretically, be hydrolysed by two alternative routes, proceeding with the fission of the bond between (a) the anomeric carbon atom and the glycosidic oxygen or (b) the aglycon group and the glycosidic oxygen. For a number of glycosides the mechanism of the acidic and/or enzymatic hydrolysis has been investigated1–8. Since it became possible to prepare highly purified glucamylase it was interesting to investigate the cleavage point when this enzyme acts on maltose.
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References
Bunton, C. A., Lewis, T. A., Llewellyn, D. R., Tristram, H., and Vernon, C. A., Nature, 174, 560 (1954).
Koshland, jun., D. E., and Stein, S. S., J. Biol. Chem., 208, 139 (1954).
Bunton, C. A., Lewis, T. A., Llewellyn, D. R., and Vernon, C. A., J. Chem. Soc., 4419 (1955).
Bunton, C. A., Silver, B. L., and Vernon, C. A., Proc. Chem. Soc., 348 (1957).
Bunton, C. A., Llewellyn, D. R., Oldham, K. G., and Vernon, C. A., J. Chem. Soc., 3588 (1958).
Mayer, F. C., and Larner, J., J. Amer. Chem. Soc., 81, 188 (1959).
Halpern, M., and Leibowitz, J., Bull. Res. Coun. Israel, A, 8, 41(1959); Biochim. et Biophys. Acta, 38, 29 (1959).
Armour, C., Bunton, C. A., Patai, S., Selman, L. H., and Vernon, C. A., J. Chem. Soc., 412 (1961).
Barker, S. A., and Fleetwood, J. G., J. Chem. Soc., 4857 (1957).
Pazur, J. H., and Ando, T., J. Biol. Chem., 234, 1966 (1959).
Bourne, E. J., Macey, A., and Peat, S., J. Chem. Soc., 882 (1945).
Oita, I. J., and Conway, H. S., Anal. Chem., 26, 600 (1954).
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FLEETWOOD, J., WEIGEL, H. Substrate Cleavage Point with Glucamylase. Nature 196, 984 (1962). https://doi.org/10.1038/196984a0
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DOI: https://doi.org/10.1038/196984a0
