Abstract
THE detection of proteolytic activity after electro-phoresis in agar gel has been described previously1,2. It has been shown with a number of hydrolytic enzymes3 that the precipitation lines formed by the antigen–antibody reaction after immunoelectrophoresis in agar gel exhibit enzymatic activity. This enzymatic activity can be demonstrated qualitatively after washing and drying the slides in the usual way by utilizing conventional histochemical techniques.
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References
Uriel, J., Nature, 188, 853 (1960).
Uriel, J., Webb, T., and Lapresle, C., Bull. Chim. Biol., 42, 1285 (1960).
Uriel, J., in Analyse Immunoelectrophoretique, by Grabar, P., and Burtin, P., 47 (Masson et Cie., 1960).
Scheidegger, J. J., Intern. Arch. Allergy App. Immunol., 7, 103 (1955).
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RAUNIO, V., GABRIEL, O. Immunoelectrophoretic Characterization of Proteolytic Enzymes. Nature 197, 1012–1013 (1963). https://doi.org/10.1038/1971012a0
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DOI: https://doi.org/10.1038/1971012a0
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