Abstract
MANY variations of the original technique of Smithies1 for starch-gel electrophoresis of proteins have been developed, notably the vertical gel2, the discontinuous buffer system3, and two-dimensional applications4,5. The latter method has been used by Ashton6 in examining pig serum proteins; it has one disadvantage in being time-consuming, requiring two electrophoretic runs, and at the most only two samples can be compared at once on normal apparatus.
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References
Smithies, O., Biochem. J., 61, 629 (1955).
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Poulik, M. D., Nature, 180, 1477 (1957).
Poulik, M. D., and Smithies, O., Biochem. J., 68, 636 (1958).
Riou, G., Paoletti, C., and Truhaut, R., Bull. Soc. Chim. Biol., 44, 149 (1962).
Ashton, G. C., Nature, 186, 991 (1960).
Miller, E. R., Ullrey, D. E., Ackerman, I., Schmidt, D. A., Hoefer, S. A., and Luecke, R. W., J. Animal Sci., 20, 31 (1961).
Ashton, G. C., J. Agric. Sci., 54, 321 (1961).
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SCOPES, R. Starch-gel Electrophoresis of Pig Serum Proteins. Nature 197, 1201 (1963). https://doi.org/10.1038/1971201a0
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DOI: https://doi.org/10.1038/1971201a0
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