Abstract
BY the method of ammonium sulphate fractionation, human plasma can be separated into a part A, which is insoluble at 33 per cent saturation, and a part B, precipitated between 33 and 45 per cent saturation. When these precipitates are dissolved in water, both the solutions spontaneously develop the ability to cause contraction of the isolated rat uterus, apparently due to the liberation of plasma kinins. From the second preparation (precipitate B) the well-characterized kinins bradykinin and kallidin have already been isolated1,2. We have now undertaken detailed investigations of the neutral ‘33 per cent precipitate’, A, with results which may be summarized as follows.
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References
Elliott, D. F., Horton, E. W., and Lewis, G. P., Biochem. J., 78, 60 (1961).
Webster, M. E., and Pierce, J. V., Conf. Structure and Function of Biologically Active Peptides: Bradykinin, Kallidin and Congeners (New York Acad. Sci, March 1962).
Armstrong, D., Jepson, J. B., Keele, C. A., and Stewart, J. W., J. Physiol., 135, 350 (1957).
Eisen, V., and Keele, C. A., J. Physiol., 154, 20 P (1960).
Croxatto, H., Pereda, T., Belmar, J., and Labarca, E., ibid. ref. 2.
Werle, E., and Maier, L., Biochem. Z., 323, 279 (1952).
Webster, M. E., and Pierce, J. V., J. Pharm. Exp. Therap., 130, 484 (1960).
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ARMSTRONG, D., MILLS, G. Chemical Characterization of Kinins of Human Plasma. Nature 197, 490 (1963). https://doi.org/10.1038/197490a0
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DOI: https://doi.org/10.1038/197490a0
