Abstract
PAPER chromatography of the gibberellins has been investigated by many investigators but no comprehensive study of all the known gibberellins has been reported. MacMillan, Seaton and Suter1 described four solvent systems which separate gibberellins A5, A6, A8 and A9 but not the pairs gibberellin A1 and gibberellic acid (A3) or gibberellins A4 and A7. The solvent systems used by Takahashi et al.2 and Phinney et al.3 do not separate gibberellins A1, A2 and A3. Gibberellins A1 and A3 can be separated on paper which is developed by overflow elution with benzene/acetic acid/water (4 : 1 : 2); this method is lengthy when conducted under gravity as described by Bird and Pugh4 and resolution is poor when development is hastened by centrifugal force5.
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References
MacMillan, J., Seaton, J. C., and Suter, P. J., Adv. Chem. Ser., 28, 18 (1961).
Takahashi, N., Kitamura, H., Kawarada, A., Seta, Y., Takai, M., Tamura, S., and Sumiki, Y., Bull. Agric. Chem. Soc. Japan, 19, 267 (1955).
Phinney, B. O., West, C. A., Ritzel, M., and Neely, P. M., Proc. U.S. Nat. Acad. Sci., 43, 398 (1957).
Bird, H. L., and Pugh, C. T., Plant Physiol., 3, 45 (1958).
Kutacek, M., Rosmus, J., and Deyl, Z., Biol. Plant, 4, 226 (1962).
Stahl, E., Chemiker Z., 82, 323 (1958); Angewandte Chem., 73, 646 (1961).
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MACMILLAN, J., SUTER, P. Thin Layer Chromatography of the Gibberellins. Nature 197, 790 (1963). https://doi.org/10.1038/197790a0
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DOI: https://doi.org/10.1038/197790a0
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