Abstract
CHITOSAN, a derivative of chitin obtained by alkaline hydrolysis, has long been known to dissolve in dilute acids and to develop a distinctive violet colour with iodine in acid medium1. A test based on these properties2 has been widely applied for the detection of chitin in arthropod cuticles and other invertebrate structures. Under undefined conditions, however, chitin has been reported to show a reluctance to display itself in the tanned exocuticle of certain myriapods3. Similarly, the hyaline exocuticle of the scorpion Pandinus imperator, though presumably containing chitin, was found to give a negative iodine–chitosan colour test4.
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MALEK, S. Chitin in the Hyaline Exocuticle of the Scorpion. Nature 198, 301–302 (1963). https://doi.org/10.1038/198301b0
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DOI: https://doi.org/10.1038/198301b0
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