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A Simplified Method for Extraction of Reduced Cytochrome-552 and -556 from Euglena gracilis (Z)

Abstract

Two spectrally different cytochromes have been isolated by chemical fractionation from Euglena gracilis (Z)1–3. Because of the differences in the absorption spectra of the chemically reduced forms, these cytochromes have been designated as cytochrome-552 and cytochrome-556. Cytochrome-552, in the reduced form, extracted from photosynthesizing green cells has absorption peaks at 552, 523, and 416 mμ, while cytochrome-556, extracted from cells grown in darkness and from Euglena mutants which are non-photosynthetic, has reduced absorption peaks at 556, 525, and 421 mμ.

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COLMANO, G., WOLKEN, J. A Simplified Method for Extraction of Reduced Cytochrome-552 and -556 from Euglena gracilis (Z). Nature 198, 783–784 (1963). https://doi.org/10.1038/198783b0

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  • DOI: https://doi.org/10.1038/198783b0

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