Negatively Stained Lipoprotein Membranes

Abstract

X-RAY diffraction studies of the air drying of myelin have included experiments in which myelin fractions from brain were dried in the presence of phosphotungstate. It is clear that this material does contain layering which electron microscopy of thin sections shows to be similar to that of the normal preparation, but it also includes additional layered systems which are significantly different. The drying of such specimens was probably appreciably slower than that achieved in the preparation of negatively stained specimens for electron microscopy, but the possibility of the existence of more than one type of layering in the latter preparations is clearly indicated.