Abstract
WITHIN the past few years nitrogen fixation has been achieved in cell-free extracts of micro-organisms including Clostridium pasteurianum, Azotobacter vinelandii, Bacillus polymyxa, and in a variety of blue-green algae1–5. We now report that cell-free lysates of A. vinelandii, prepared by adding A22 phage6 to cultures of the bacteria during early exponential growth, incorporate nitrogen gas.
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NIMECK, M., WILSON, P. & NICHOLAS, D. Nitrogen Fixation in Cell-free Extracts of Azotobacter vinelandii prepared by Lysis with Phage A22. Nature 200, 709 (1963). https://doi.org/10.1038/200709a0
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DOI: https://doi.org/10.1038/200709a0
