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Cultivation of Horsesickness Virus in Tissue Culture

Nature volume 200, page 716 (1963)Cite this article

Abstract

Nieschulz1 and Alexander2 independently showed that horsesickness virus multiplies in the brains of Swiss albino mice. Alexander3 reported later the successful cultivation of mouse-adapted horsesiekness virus in the brains of guinea pigs, rats and other rodents. He also succeeded in cultivating a mouse-adapted strain of virus in embryonated hen's eggs, in which multiplication of the virus occurred in the brain of the embryo without producing any specific mortality4. McIntosh5 propagated viscero-tropic horsesickness virus strains in 8-day-old embryonated hen's eggs at an optimum temperature of 32° C. Embryo mortality occurred regularly during the initial passages.

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References

  1. Nieschulz, O., Tydsch. Diergeneeskunde, 59, 1433 (1932).

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  2. Alexander, R. A., J.S. Afr. Vet. Med. Assoc., 4, 1 (1933).

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  3. Alexander, R. A., Onderstepoort J. Vet. Sti. Anim. Ind., 4, 291 (1935).

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  4. Alexander, R. A., Onderstepoort J. Vet. Sci. Anim. Ind., 11, 9 (1938).

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  5. McIntosh, B. M. (unpublished observations).

  6. Reed, L. J., and Muench, H., Amer. J. Hyg., 27, 493 (1938).

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  7. McIntosh, B. M., Onderstepoort J. Vet. Res., 27, 165 (1956).

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Authors and Affiliations

  1. Section of Virology, Veterinary Research Institute, Onderstepoort, South Africa

    B. J. ERASMUS

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  1. B. J. ERASMUS
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ERASMUS, B. Cultivation of Horsesickness Virus in Tissue Culture. Nature 200, 716 (1963). https://doi.org/10.1038/200716a0

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  • DOI: https://doi.org/10.1038/200716a0

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