Abstract
THE thin-layer chromatographic separation of steroids on silica-gel chromatoplates is a rapid and useful preparative and analytical technique1–4. However, the ascending organic solvent development of chromatoplates in an atmosphere of variable temperature and humidity produces streaking and poor resolution of C-19 and C-21 steroids. This communication describes a method which eliminates these difficulties.
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COHN, G., PANCAKE, E. A Standardized Technique for the Separation of Steroids by Thin-layer Chromatography. Nature 201, 75–76 (1964). https://doi.org/10.1038/201075b0
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DOI: https://doi.org/10.1038/201075b0
