Abstract
IN the development of growing plant cell suspensions from callus cultures, there is considerable cell agglomeration for the first 15–20 generations; under our growth conditions1 this represents 4–5 sub-cultures. The diameter of the cell agglomerates may be as high as 0.5 cm, thus transfer by pipette is difficult.
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References
Speake, T., McCloskey, P., Smith, W. K., Scott, T. A., and Hussey, H., Nature, 201, 614 (1964).
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SCOTT, T., HUSSEY, H. & SKELTON, C. A Device for the Transfer of Suspended Tissue Cultures. Nature 201, 742 (1964). https://doi.org/10.1038/201742a0
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DOI: https://doi.org/10.1038/201742a0
