Abstract
THE enzymatic reaction catalysed by glyceraldehyde-3-phosphate dehydrogenase (GAPD) is thought to occur through the formation of an acyl-enzyme compound which involves some of the thiol groups of the enzyme1–3. Although the complex nature of enzyme reactions renders it improbable to attribute the whole process to the functioning of one single type of group, no experimental evidence has so far been supplied concerning other residues essential for GAPD action. In order to obtain more information in this field we adopted the photo-oxidation technique as described by Weil et al.4.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on SpringerLink
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Racker, E., and Krimsky, I., Nature, 169, 1043 (1952).
Velick, S. F., and Hayes, jun., J. E., J. Biol. Chem., 203, 545 (1953).
Koeppe, O. J., Boyer, P. D., and Stulberg, M. P., J. Biol. Chem., 219, 569 (1956).
Weil, L., Gordon, W. G., and Buchert, A. R., Arch. Biochem. Biophys., 33, 90 (1954).
Szörényi, E. T., and Elödi, P., Ukr. Biokhim. Zh., 26, 387 (1954).
Moore, S., Spackman, D. H., and Stein, W. H., Anal. Chem., 30, 1185 (1958).
Ray, jun., W. J., and Koshland, jun., D. E., J. Biol. Chem., 236, 1973 (1961).
Szabolcsi, G., and Biszku, E., Biochim. Biophys. Acta, 48, 335 (1961).
Brake, J. M., and Wold, F., Biochim. Biophys. Acta, 40, 171 (1960).
Robinson, D., and Stollar, D., Fed. Proc., Abst. Forty-sixth Ann. Meeting, 232, f (1962).
Elödi, P., Acta Physiol. Hung., 13, 199 (1958).
Boyer, P. D., J. Amer. Chem. Soc., 76, 4331 (1954).
Horecker, B. L., and Kornberg, A. A., J. Biol. Chem., 175, 385 (1948).
Szabolcsi, G., Biszku, E., and Szörényi, E., Biochim. Biophys. Acta, 35, 237 (1959).
Velick, S. F., J. Biol. Chem., 203, 563 (1953).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
FRIEDRICH, P., POLGÁR, L. & SZABOLCSI, G. Effect of Photo-oxidation on Glyceraldehyde-3-phosphate Dehydrogenase. Nature 202, 1214–1215 (1964). https://doi.org/10.1038/2021214a0
Issue date:
DOI: https://doi.org/10.1038/2021214a0
