Eosinophil Leucocyte Reaction to Tricycloquina-zoline-treated Cells and to this Chemical

Abstract

SUBCUTANEOUS implantation of isolated cell suspensions in mice gave a marked local eosinophil leucocyte reaction when the cells were of homologous and heterologous origin, but not when isologous normal cells or tumour cells of either native or foreign origin were used¹. Implantation of isologous liver cells prepared from mice treated in vivo with hepatocarcinogenic compounds 3′-methyl-4-dimethyl-aminoazobenzene or o-aminoazotoluene resulted in a marked local eosinophil leucocyte reaction similar to that produced by the inoculation of genetically dissimilar cells². It was suggested that the tissue specific antigen (TSA) of either foreign (homologous or heterologous) or native (isologous) cells, which had been altered or modified by the carcinogens, acted as ‘foreign’ within the isologous host and thus evoked a local eosinophil leucocyte reaction under these experimental conditions. Tumour cells lacking in TSA^3–5 failed to evoke a similar reaction even though there was a much greater genetic diversity between the tumour and the host. Further experiments described here were performed to throw some light on the aforementioned hypothesis.