Stages in the Formation of Yeast Protoplasts with Strepzyme

Abstract

IN a continuation of our work on the mode of action of the enzyme preparation obtained from, the cell-free supernatant of Streptomyces GM, so called ‘strepzyme’^1,2, we have found that, with our culture conditions, the cell-walls of Candida utilis are highly susceptible to enzyme action. The aim of the experiments recorded here was to ascertain whether the process of cell-wall digestion with this lytic enzyme preparation runs parallel to that observed when using the digestive juice of the snail Helix pomatia.