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Efficient Labelling of Tissue-soluble Proteins with Iodine-131 using Chloramine T

Nature volume 203, pages 985–986 (1964)Cite this article

Abstract

FOLLOWING the reports of Hunter and Greenwood1 and Reith and Tampion2, the chloramine T radioiodination procedure has been applied3,4 for labelling, with iodine-131, serum proteins from several animal species. High labelling efficiency (about 65 per cent) could be obtained provided oxidation was allowed to proceed for at least 30 min. The advantage of using a protein: chloramine T ratio of about 250 : 1 has been stressed3,4 in order to avoid even small damage to the proteins, undetectable by electrophoretic and immunological analyses but evident from metabolic behaviour in vivo.

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References

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  1. Istituto di Fisiologia Generale, Via Laterina, 4, Siena, Italy

    V. BOCCI

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  1. V. BOCCI
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BOCCI, V. Efficient Labelling of Tissue-soluble Proteins with Iodine-131 using Chloramine T. Nature 203, 985–986 (1964). https://doi.org/10.1038/203985a0

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