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Effect of the Implantation Site on the Development of Grafted Limb Bud Mesoderm in Chick Embryos

Abstract

A SMALL rectangular block of the mesoderm immediately subjacent to the superficial venous plexus was excised from the wing bud apex in stages 25–26 of chick embryos and replaced with an isolate of mesoderm from the prospective thigh or proximal foreleg region. The graft extended from the dorsal to the ventral surface of the wing bud apex and did not lie in contact with the apical ridge of the ectoderm; it was separated from the latter structure by the mesoderm intervening between the apical ectoderm and the superficial venous plexus, namely, by about 15–20 layers of mesodermal cells of the wing bud (histological control).

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AMPRINO, R., BONETTI, D. Effect of the Implantation Site on the Development of Grafted Limb Bud Mesoderm in Chick Embryos. Nature 204, 298 (1964). https://doi.org/10.1038/204298a0

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