Abstract
ORGANISMS which fix carbon dioxide can be used to advantage for the preparation of radioactive labelled compounds. During growth, radioactive carbon from 14CO2 can be incorporated into all cellular substances. If growth is inhibited, assimilated carbon is bound primarily in the form of reserve material. Several ‘knallgas’ bacteria of the Hydrogenomonas type incorporate 14CO2 into poly-β-hydroxybutyric acid (PHBA) in a hydrogen–oxygen atmosphere in the absence of a source of nitrogen1,2. PHBA can be depolymerized to D(—)-β-hydroxybutyric acid (HBA) by using either hydrazine3 or enzymatic hydrolysis4,5.
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References
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GOTTSCHALK, G., SCHLEGEL, H. Preparation of 14C-D(—)-β-hydroxybutyric Acid from 14CO2 using ‘Knallgas’ Bacteria (Hydrogenomonas). Nature 205, 308 (1965). https://doi.org/10.1038/205308a0
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DOI: https://doi.org/10.1038/205308a0
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