Diffusion of Carbon Dioxide through Thin Layers of Solution

Abstract

WE have adapted the Millipore filter principle of Scholander and Hemmingsen^1,2 to an extremely simple apparatus, and have used this to investigate the diffusion of carbon dioxide through buffer solutions with and without carbonic anhydrase. While the accuracy of absolute determinations of diffusion coefficient using this method is limited by uncertainty as to the thickness of the layer of fluid, the porosity of the Millipore filter, and the precise [CO₂] gradient, it is clearly an adequate method for demonstrating large ratios of fluxes.